Shanghai Sonyt Biotechnology Co., Ltd. is one of the leading manufacturers and suppliers of biotinyl-(arg8)-vasopressin in China, also supports custom service. Welcome to wholesale bulk high quality biotinyl-(arg8)-vasopressin from our factory. Contact us for more details.
| Description | Biotinyl-(Arg8)-Vasopressin is the N-biotinylated nine-residue hypothalamic neurohypophyseal hormone arginine vasopressin (ADH), with a Cys1-Cys6 disulfide loop. It agonizes V1a (vasoconstriction) and V2 (water reabsorption) receptors; the biotin tag enables streptavidin-based detection, immobilization or receptor-affinity media. |
| CAS No. | 126703-17-1 |
| Molecular weight (Da) | ~1280 (biotinylated, disulfide) |
| Structure type | Cyclic 9-mer (arginine vasopressin, C-amide, N-biotinylated) |
| Solubility | Readily soluble in water/PBS |
| Storage | Powder, −20°C, dry, light-protected |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| Biotinyl-(Arg8)-Vasopressin (Biotin-Cys-Tyr-Phe-Gln-Asn-Cys-Pro-Arg-Gly-NH2, Cys1-Cys6 disulfide) | Biotin-AVP; Biotinyl-ADH; biotinylated arginine vasopressin; biotinylated antidiuretic hormone; CAS 126703-17-1 | biotinylated peptide, neuropeptide, arginine vasopressin (AVP/ADH), V1a/V2 receptor probe, disulfide-containing ring, streptavidin pull-down, water homeostasis/osmoregulation, reductant-sensitive | Nine-residue cyclic-linear hybrid: Biotin-Cys1-Tyr-Phe-Gln-Asn-Cys6-Pro-Arg-Gly-NH2; intramolecular Cys1-Cys6 disulfide (cystine, net -2H) forms a 20-membered ring (Cys1-Tyr-Phe-Gln-Asn-Cys6); C-terminal Gly amidated (-CONH2); N-terminal Cys1 alpha-amine coupled to biotin via amide bond. Biotin net addition C10H14N2O2S; contains 1 Tyr, 1 Phe, 1 Pro, 1 Arg (side-chain guanidinium +1); the disulfide ring is essential for receptor agonism; biotin is conjugated at the N-terminal Cys alpha-amine (not on a side chain, not at Gln4), preserving the native AVP agonistic conformation | C56H79N17O14S3 | 1309.52 | 1310.53 | Free base / TFA or acetate exchangeable (per request); contains Cys1-Cys6 intramolecular disulfide; CAS 126703-17-1 | 9 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-BioAVP-01 | White to off-white powder | Store at -20°C, sealed, desiccated, under argon/nitrogen, ~2 years; aliquot to avoid repeated freeze-thaw. STRICTLY AVOID reducing agents (DTT, TCEP, beta-mercaptoethanol, excess cysteine) - reduction of the Cys1-Cys6 disulfide opens the ring and abolishes V1a/V2 agonistic activity. | 2-8°C for ~2 weeks; working aliquots at -20°C, prepare fresh. | Short peptide with good water solubility (Arg guanidinium, amidated C-terminus, polar disulfide ring); dissolve directly in water or pH 4-6 acetate/citrate buffer. Do NOT use DTT/TCEP. Biotin forms a high-affinity noncovalent complex with streptavidin (Kd ~1e-14 M), enabling V1a/V2 pull-down, affinity purification and ELISA tracing. Use working solutions within 24 h to prevent disulfide exchange or aggregation. | solid stable ~2 years at -20°C, sealed, desiccated, under inert gas; the disulfide is stable dry but must be protected from moisture and strong acid/base. |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Pathways |
| PEP-BioAVP-01 | N-terminally biotinylated arginine vasopressin (AVP/ADH) nonapeptide analogue retaining the Cys1-Cys6 disulfide ring and C-terminal amide-used for V1a/V2 receptor binding, signaling studies and streptavidin pull-down. | Arginine vasopressin (AVP, also antidiuretic hormone ADH) is a nonapeptide neuropeptide synthesized in hypothalamic supraoptic/paraventricular nuclei and released from the neurohypophysis, acting through three GPCRs: V1aR (vascular smooth muscle/liver/platelets, PI-Ca2+ pathway, mediates vasoconstriction and hepatic glycogenolysis), V1bR/V3R (pituitary ACTH secretion) and V2R (renal collecting-duct principal cells, cAMP-PKA pathway, mediates water reabsorption/antidiuresis). Native AVP sequence is Cys1-Tyr-Phe-Gln-Asn-Cys6-Pro-Arg8-Gly9-NH2; the Cys1-Cys6 disulfide ring and C-terminal Gly-NH2 are essential for high-affinity receptor binding and agonism. This product conjugates biotin to the N-terminal Cys1 alpha-amine without disrupting the disulfide ring or C-terminal amidation, preserving full agonistic activity and affinity at V1a/V2 receptors while adding streptavidin high-affinity binding (Kd ~1e-14 M). Major applications: (1) biotin-streptavidin pull-down to capture V1aR/V2R from membrane lysates or tissue homogenates for receptor identification, affinity purification and expression profiling; (2) streptavidin-HRP/fluorophore ELISA or radioligand binding to quantify receptor density and ligand competition; (3) receptor affinity and signaling studies (V1a IP3/Ca2+, V2 cAMP); (4) models of dysregulated AVP pathways (diabetes insipidus, SIADH, heart failure, cirrhosis, hypotension/shock); (5) in vitro competition ligand for V2 antagonists (tolvaptan) and V1a agonists in drug discovery. CRITICAL CONTRAINDICATION: the disulfide ring is the activity core-never use DTT, TCEP, beta-mercaptoethanol or excess free cysteine/reduced glutathione, as reduction opens the ring and irreversibly abolishes receptor affinity and agonism. | Water homeostasis/antidiuretic hormone research, V1a/V2/V1b receptor pharmacology, neuroendocrinology, hypertension/heart failure/cirrhosis, diabetes insipidus/SIADH, biotin-streptavidin pull-down, receptor affinity purification, GPCR signaling (cAMP/IP3/Ca2+), tolvaptan/V2 antagonist in vitro screening | V1a receptor (AVPR1A, Gq/11-PLC-IP3-Ca2+), V2 receptor (AVPR2, Gs-cAMP-PKA), V1b/V3 receptor (AVPR3, ACTH secretion); high-affinity binding to streptavidin/avidin |
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