Fluorescent label

 

Fluorescent dyes are important tools for biomolecule labeling. Fluorescein derivatives can be widely labeled on reagents used in fluorescence microscopy, flow cytometry, and immunofluorescence detection. Typically, biotin, FITC Such dyes can be arbitrarily labeled on peptides N terminal or C end. But we recommend N End marking has a higher success rate, shorter time, and easier operation. Since peptides are derived from C end to N end synthesis, so N End modification will be the final step in the solid-phase synthesis, eliminating the need for additional coupling steps. On the contrary, if it is C End tagging requires additional steps and the process is more complex.

 

1727320167214663

 

Ordinary fluorescence modification

Fluorescence modification Chinese name N end N End with linker
Biotin-labeled peptides Biotin- Biotin-Ahx-
fluorescein isothiocyanate FITC- FITC-Ahx-
5-carboxyfluorescein 5-FAM- 5-FAM-Ahx-
dansylfluorescein Dansyl- Dansyl-Ahx-
5-carboxytetramethylrhodamine TMR- (TAMRA-) TMR-Ahx- (TAMRA-Ahx-)

 

Pass Lys Fluorescence modification of side chain amino linkages

polypeptide N end middle polypeptide sequence polypeptide C end
Lys(Biotin)- -Lys(Biotin)- -Lys(Biotin)
Lys(FITC)- -Lys(FITC)- -Lys(FITC)
Lys(5-FAM)- -Lys(5-FAM)- -Lys(5-FAM)
Lys(Dansyl)- -Lys(Dansyl)- -Lys(Dansyl)
Lys(TMR)- -Lys(TMR)- -Lys(TMR)
Lys(Dnp)- -Lys(Dnp)- -Lys(Dnp)

 

The excitation light wavelength and emission light wavelength of the fluorescent materials that our company often produces

Fluorophore Ex(nm) Em(nm) Fluorophore Ex(nm) Em(nm)
Hydroxycoumarin 325 386 R-phycoerythrin (PE) (489) 565 578
Dansyl chloride 340 578 Rhodamine Red-X 560 580
AMC 345 445 Tamara 565 580
Methoxycoumarin 360 410 Alexa fluor 555 556 573
Alexa fluor series 345 442 Alexa fluor 546 556 573
Aminocoumarin 350 445 Rox 575 602
Dabcyl 453 - Alexa fluor 568 578 603
Cy2 490 510 Texas Red 589 615
FAM 495 517 Alexa fluor 594 590 617
Alexa fluor 488 494 517 Alexa fluor 621 639
FITC 495 519 Alexa fluor 633 650 668
Alexa fluor 430 430 545 Cy5 (625) 650 670
5-FAM 492 518 Alexa fluor 660 663 690
Alexa fluor 532 530 555 Cy5.5 675 694
HEX 535 556 TruRed 490; 675 695
5-TAMRA 542 568 Alexa fluor 680 679 702
Cy3 550 570 Cy7 743 767
TRITC 547 572 Cy3.5 581 596