Fluorescent label
Fluorescent dyes are important tools for biomolecule labeling. Fluorescein derivatives can be widely labeled on reagents used in fluorescence microscopy, flow cytometry, and immunofluorescence detection. Typically, biotin, FITC Such dyes can be arbitrarily labeled on peptides N terminal or C end. But we recommend N End marking has a higher success rate, shorter time, and easier operation. Since peptides are derived from C end to N end synthesis, so N End modification will be the final step in the solid-phase synthesis, eliminating the need for additional coupling steps. On the contrary, if it is C End tagging requires additional steps and the process is more complex.

Ordinary fluorescence modification
| Fluorescence modification Chinese name | N end | N End with linker |
| Biotin-labeled peptides | Biotin- | Biotin-Ahx- |
| fluorescein isothiocyanate | FITC- | FITC-Ahx- |
| 5-carboxyfluorescein | 5-FAM- | 5-FAM-Ahx- |
| dansylfluorescein | Dansyl- | Dansyl-Ahx- |
| 5-carboxytetramethylrhodamine | TMR- (TAMRA-) | TMR-Ahx- (TAMRA-Ahx-) |
Pass Lys Fluorescence modification of side chain amino linkages
| polypeptide N end | middle polypeptide sequence | polypeptide C end |
| Lys(Biotin)- | -Lys(Biotin)- | -Lys(Biotin) |
| Lys(FITC)- | -Lys(FITC)- | -Lys(FITC) |
| Lys(5-FAM)- | -Lys(5-FAM)- | -Lys(5-FAM) |
| Lys(Dansyl)- | -Lys(Dansyl)- | -Lys(Dansyl) |
| Lys(TMR)- | -Lys(TMR)- | -Lys(TMR) |
| Lys(Dnp)- | -Lys(Dnp)- | -Lys(Dnp) |
The excitation light wavelength and emission light wavelength of the fluorescent materials that our company often produces
| Fluorophore | Ex(nm) | Em(nm) | Fluorophore | Ex(nm) | Em(nm) |
| Hydroxycoumarin | 325 | 386 | R-phycoerythrin (PE) (489) | 565 | 578 |
| Dansyl chloride | 340 | 578 | Rhodamine Red-X | 560 | 580 |
| AMC | 345 | 445 | Tamara | 565 | 580 |
| Methoxycoumarin | 360 | 410 | Alexa fluor 555 | 556 | 573 |
| Alexa fluor series | 345 | 442 | Alexa fluor 546 | 556 | 573 |
| Aminocoumarin | 350 | 445 | Rox | 575 | 602 |
| Dabcyl | 453 | - | Alexa fluor 568 | 578 | 603 |
| Cy2 | 490 | 510 | Texas Red | 589 | 615 |
| FAM | 495 | 517 | Alexa fluor 594 | 590 | 617 |
| Alexa fluor 488 | 494 | 517 | Alexa fluor | 621 | 639 |
| FITC | 495 | 519 | Alexa fluor 633 | 650 | 668 |
| Alexa fluor 430 | 430 | 545 | Cy5 (625) | 650 | 670 |
| 5-FAM | 492 | 518 | Alexa fluor 660 | 663 | 690 |
| Alexa fluor 532 | 530 | 555 | Cy5.5 | 675 | 694 |
| HEX | 535 | 556 | TruRed | 490; 675 | 695 |
| 5-TAMRA | 542 | 568 | Alexa fluor 680 | 679 | 702 |
| Cy3 | 550 | 570 | Cy7 | 743 | 767 |
| TRITC | 547 | 572 | Cy3.5 | 581 | 596 |