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| Product name | CAS:923949-90-0;Biotin-Crosstide |
| Sequence | {Biotin}-GRPRTSSFAEG |
| Three letter sequence | {Biotin}-Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly |
| Peptide purity (HPLC, %) | 95%/99% |
| Molecular formula | C58H91N19O19S |
| Molecular weight (Da) | 1390.52 |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Amino Acid Full Name Sequence | Molecular Formula | Exact Molecular Weight (Da) | Average Molecular Weight (Da) | Terminal Modification - N-terminus | Terminal Modification - C-terminus | Sequence Length (aa) |
| Biotin-Crosstide | #Biotin-Crosstide #AKT kinase fluorogenic substrate; #Biotinylated kinase peptide; #In vitro PKB detection substrate | AKT/PKB kinase activity assay, protein phosphorylation assay, high-throughput kinase inhibitor screening, immunoblot capture, biotin affinity chromatography, cellular signaling pathway research | Biotin-Glycine-Arginine-Proline-Serine-Serine-Leucine-Serine-Threonine | C₅₃H₉₃N₁₉O₁₆S | 1283.684 | 1284.48 | Biotin modification at N-terminus | Free carboxyl group | 9 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage Conditions | Short-term Storage Conditions | Liquid Stability | Solid Powder Stability |
| PEP-BIO-CRO01 | White powder | -20℃, light-proof sealed, light-resistant aliquot packaging to avoid biotin photodegradation, prevent repeated freeze-thaw | Store at 2-8℃ away from light, use up within 1 month, prepare fresh before use | Stable for 24 h at 4℃ without light; prone to degradation under illumination | Stable for 3 years at -20℃ light-proof sealed, 2 years at 4℃ light-proof |
Functional Description
| Product Code | Frontend Short Summary (for search list display) | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Target / Receptor |
| PEP-BIO-CRO01 | Biotin-labeled Crosstide, specific phosphorylation substrate for AKT/PKB, applied in kinase activity detection, inhibitor screening and affinity enrichment assays via biotin tag | Biotin-Crosstide is classic N-terminally biotinylated peptide substrate specific for AKT (PKB), containing Ser/Thr phosphorylation sites recognized by AKT kinase. Activated kinase catalyzes serine/threonine phosphorylation on this substrate. N-terminal biotin enables high specific binding to streptavidin for ELISA, AlphaLISA, affinity pull-down and Western Blot capture assays. Widely used for quantitative in vitro AKT kinase activity, high-throughput screening of PI3K/AKT pathway inhibitors, phosphoprotein enrichment and research on tumor cell signaling transduction, suitable for molecular pharmacology, kinase drug screening and cellular signal pathway laboratories | Molecular pharmacology, AKT/PKB kinase assay, PI3K signaling pathway, kinase inhibitor screening, phosphoprotein enrichment, biotin affinity assay | AKT/PKB protein kinase, streptavidin, phospho-Ser/Thr antibody, downstream targets of PI3K pathway |
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