Shanghai Sonyt Biotechnology Co., Ltd. is one of the leading manufacturers and suppliers of biotin-ova (323-339) in China, also supports custom service. Welcome to wholesale bulk high quality biotin-ova (323-339) from our factory. Contact us for more details.
| Description | OVA(323-339) is the canonical I-Ad-restricted MHC-II epitope recognized by OVA-specific CD4⁺ helper T cells. The biotin tag enables streptavidin multimerization into MHC-II tetramers for detection/quantification of antigen-specific CD4⁺ T cells in vaccination and immunity studies. |
| Structure | Linear 17-mer (OVA 323-339 ISQAVHAAHAEINEAGR, N-biotinylated) |
| Solubility | Readily soluble in water/PBS |
| Storage | Powder, −20°C, dry, light-protected |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| Biotin-Ovalbumin (323-339) [N-terminally biotinylated ISQAVHAAHAEINEAGR] | Biotin-OVA(323-339); Biotin-ISQAVHAAHAEINEAGR; OVA 323-339 I-Ad Th epitope; N-biotinyl-OVA(323-339); biotinylated ovalbumin Th epitope peptide | MHC-II epitope peptide, OVA(323-339), I-Ad restricted, CD4 Th epitope, biotin label, DO11.10 hybridoma, streptavidin pull-down, tetramer loading | Linear 17-mer, sequence ISQAVHAAHAEINEAGR (chicken ovalbumin residues 323-339); biotin (biotinoyl, C10H16N2O3S dehydrated) conjugated via an amide bond to the N-terminal alpha-amine of Ile; C-terminal free Arg-OH; the N-terminal alpha-amine is acylated and carries no positive charge; net charge approximately -1 to 0 (Arg side chain +1, Glu side chain -1, C-terminus -1, His partially protonated at pH 7); contains 1 His (pH-sensitive), 1 Gln, 2 Ala, 2 Ile; the biotin bicyclic imidazolidine-tetrahydrothiophene moiety binds streptavidin/avidin with ultra-high affinity (Kd ~10^-14 M). Sequence source: chicken ovalbumin (UniProt P01012) residues 323-339. | C84H134N28O27S | 1998.97 | 2000.22 | Free acid (C-terminal -COOH); TFA/acetate forms available on request | 17 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-BOVA323-01 | White to off-white powder (biotin has no strong chromophore) | Store at -20°C, sealed, desiccated, ~2 years. Aliquot to avoid repeated freeze-thaw. | 2–8°C for up to ~2 weeks; working aliquots at -20°C; ship on blue ice at ambient temperature. | Readily soluble in water or neutral buffer (PBS pH 7.4, HBSS); prepare 1–10 mg/mL stock, filter-sterilize through 0.22 μm, and aliquot. Reconstituted solution stable for ~1 week at 2–8°C and ~3 months at -20°C; avoid repeated freeze-thaw. The biotin tag remains stably bound to streptavidin and can be used directly for in vitro MHC-II tetramer refolding/loading or pull-down assays. | solid stable for ~2 years at -20°C, sealed, desiccated; no light-sensitive groups; brief room-temperature shipping is acceptable. |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Pathways |
| PEP-BOVA323-01 | N-terminally biotinylated OVA(323-339), the classic MHC-II I-A^d-restricted CD4 Th epitope (ISQAVHAAHAEINEAGR), for DO11.10 OVA-specific T-cell hybridoma / transgenic T-cell activation, MHC-II tetramer loading, and streptavidin pull-down studies. | Ovalbumin (OVA) is the major egg-white protein; residues 323-339 (core nonamer QAVHAAHAEINEAGR) constitute the most widely used CD4+ helper T-cell epitope presented by the mouse MHC-II molecule I-A^d (H-2^d haplotype, BALB/c mice). The N-terminally biotinylated version retains I-A^d binding while enabling, through the high-affinity biotin-streptavidin interaction (Kd ~10^-14 M): (1) in vitro MHC-II-peptide tetramer assembly-biotinylated peptide is refolded into I-A^d and cross-linked with fluorophore-conjugated streptavidin for flow cytometry/staining of OVA-specific CD4 T cells; (2) streptavidin magnetic-bead/resin pull-down to enrich pMHC-II complexes or interacting proteins from cell lysates; (3) immunoprecipitation and pMHC-complex purification; (4) antigen presentation and activation assays using the DO11.10 OVA-specific T-cell hybridoma (whose TCR recognizes OVA 323-339/I-A^d), with readouts of IL-2/IFN-γ secretion; (5) loading peptide for OT-II (OVA 323-339-specific CD4 TCR transgenic) mouse experiments; (6) model Th epitope in vaccine adjuvant/delivery studies. **Critical**: this epitope is strictly I-A^d-restricted and is presented only by BALB/c (H-2^d) or I-A^d-expressing antigen-presenting cells; the DO11.10 hybridoma recognizes the same epitope. | MHC-II epitope, CD4 Th-cell activation, OVA model antigen, DO11.10 hybridoma, OT-II transgenic T cells, MHC-II tetramer, streptavidin pull-down, immunoprecipitation, vaccine model epitope, antigen presentation research | Mouse MHC-II molecule I-A^d (H-2^d); DO11.10 TCR; biotin binds streptavidin/avidin (Kd ~10^-14 M) |
Hot Tags: biotin-ova (323-339), China biotin-ova (323-339) manufacturers, suppliers, factory
