Shanghai Sonyt Biotechnology Co., Ltd. is one of the leading manufacturers and suppliers of 5-fam-β-amyloid (1-42) human tris in China, also supports custom service. Welcome to wholesale bulk high quality 5-fam-β-amyloid (1-42) human tris from our factory. Contact us for more details.
| Description | Aβ1-42 conjugated to 5-FAM green fluorophore for fluorescence monitoring of aggregation kinetics, fibril binding, cellular uptake and membrane interactions; a common in-vitro imaging tracer. |
Product Basic Information Table
| Standard English Name | Abbreviations/Aliases | Functional Tags | Structural Core | Molecular Formula | Exact MW (Da) | Average MW (Da) | Salt Form | Length (aa) |
| 5-FAM-β-Amyloid (1-42), human Tris salt (human Aβ(1-42) N-terminally conjugated to 5-carboxyfluorescein; green fluorescent probe; Tris salt) | 5-FAM-Aβ(1-42); FAM-labeled Aβ42; 5-carboxyfluorescein Aβ42; 5-FAM-Aβ42 | β-amyloid peptide, 5-FAM fluorescent label, green fluorescent probe (Ex492/Em518), light-sensitive / protect from light, aggregation kinetics, cell imaging, FCS/FRET, live-cell tracing | Linear 42-mer; human Aβ(1-42) N-terminal Asp1 covalently linked via amide bond to 5-carboxyfluorescein (5-FAM); λex≈492 nm / λem≈518 nm (green fluorescence); retains full Aβ42 aggregation, oligomerization and fibrillization capacity | C224H322N55O67S1 | 4886.32 | 4889.41 | Tris-buffered salt ( powder; protect from light at all steps) | 42 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Stability |
| PEP-FAB142T-01 | Yellow to yellow-green powder (characteristic 5-FAM fluorescent tint; solid is visibly fluorescent) | Store at −20°C, sealed, desiccated, STRICTLY protected from light (wrapped in aluminium foil / amber microcentrifuge tubes); stable ~1–2 years. Aliquot into single-use portions to avoid repeated freeze-thaw. Avoid prolonged exposure to bright light at room temperature. | 2–8°C protected from light for ~1 week; liquid working solutions must be kept in amber tubes or wrapped in foil at all times; use on the same day when possible. | Relatively water-soluble (N-terminal 5-FAM carries a carboxylate and improves overall solubility); dissolves in water or neutral buffer. However, liquid state still aggregates according to intrinsic Aβ42 kinetics - pretreat with the standard HFIP/DMSO protocol and use freshly prepared solutions. PROTECT FROM LIGHT at every step: illumination causes 5-FAM photobleaching and reduces quantum yield. Avoid repeated freeze-thaw. | solid stable for ~1–2 years at −20°C, desiccated and light-protected. 5-FAM is light- and oxygen-sensitive; store dry, under inert gas, in a sealed amber container. Minimize light exposure even during weighing/aliquoting. |
Functional Description
| Product Code | Short Summary (listing) | Detailed Biological Function | Research Areas | Targets/Receptors |
| PEP-FAB142T-01 | Human Aβ(1-42) N-terminally conjugated to 5-carboxyfluorescein (5-FAM) green-fluorescent probe (42-mer, Tris salt; Ex492/Em518 nm). Labeled Aβ retains full aggregation, oligomerization and fibrillization capacity, enabling real-time tracking of Aβ in cells, tissues or in vitro by fluorescence microscopy, confocal, FCS/FRET and fluorescence polarization - used for aggregation-inhibitor screening, cellular uptake and co-localization studies. | 5-FAM-Aβ(1-42) is a fluorescent tracer obtained by covalently coupling green-emitting 5-carboxyfluorescein (5-FAM, λex/em = 492/518 nm) via an amide bond to N-terminal Asp1 of full-length human Aβ(1-42). Compared with radiolabeling or residue-specific point-mutation tags, N-terminal 5-FAM modification has minimal impact on Aβ aggregation propensity, secondary structure, oligomerization and fibril elongation, making it the preferred tracer for in vitro and cellular aggregation kinetics. Typical applications include: (1) real-time monitoring of the nucleation–elongation–plateau curve in parallel with ThT or fluorescence polarization; (2) FRET/FCS (fluorescence correlation spectroscopy) to measure oligomer size distribution and diffusion coefficients; (3) live-cell confocal tracing of Aβ uptake, endocytic routes and subcellular co-localization with lysosomal, mitochondrial or ER markers in neurons, microglia and astrocytes; (4) high-throughput screening of aggregation inhibitors (small molecules, peptides, antibodies, chaperones) - 5-FAM-Aβ42 is typically mixed with unlabeled Aβ42 at ~1:9 to 1:20, and inhibition read out by fluorescence anisotropy or FRET; (5) in vitro membrane-binding and liposome-perturbation assays. Practical notes: high label ratio alters aggregation behavior - keep labeled:unlabeled ≤ 1:10; keep samples on ice and protected from light to minimize photobleaching; fluorescence readout is sensitive to buffer pH, residual DMSO and adsorption to glass surfaces. | Alzheimer's disease (AD), Aβ aggregation fluorescent tracking, live-cell imaging, FRET/FCS aggregation kinetics, high-throughput aggregation-inhibitor screening, cellular uptake and endocytosis, subcellular co-localization, fluorescence polarization (FP) assays | Aβ self-aggregation (monomer → oligomer → fibril); fluorescence channel Ex≈492 nm / Em≈518 nm (compatible with FITC/GFP filter sets) |
Hot Tags: 5-fam-β-amyloid (1-42) human tris, China 5-fam-β-amyloid (1-42) human tris manufacturers, suppliers, factory, Amyloid 1 28 , 186359-65-9, 190436-05-6, CAS 152286 31 2, CAS 190436 05 6, CAS 659739 33 0
