![[Glu1]-Fibrinopeptide B](/uploads/48442/glu1-fibrinopeptide-b471c1.jpg)
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| Description | [Glu1]-Fibrinopeptide B (CAS: 103213-49-6) is the position-1 variant of human fibrinopeptide B: the N-terminal pyroglutamic acid (pGlu) of the native FPB is replaced by glutamic acid (Glu), sequence Glu-Gly-Val-Asn-Asp-Asn-Glu-Glu-Gly-Phe-Phe-Ser-Ala-Arg. Molecular formula C66H95N19O26, MW 1570.57 Da, approximately +18 Da heavier than native FPB. The free N-terminal Glu gives predictable MS/MS fragmentation, making [Glu1]-FPB a widely used internal standard for mass-spectrometric quantification of thrombin cleavage products (e.g., in SISCAPA and MRM assays for FPB), and a tool for studying the effects of N-terminal capping on peptide conformation and thrombin recognition. |
| Structure type | Linear 14-mer (N-terminal free Glu human FPB position-1 variant) |
| Solubility | Readily soluble in water, PBS buffer |
| Storage | Powder -20C, dry, protected from light |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| [Glu1]-Fibrinopeptide B (N-terminal free-Glu linear analog of human fibrinopeptide B; a widely used MS internal standard/calibrant peptide) | [Glu1]-Fibrinopeptide B; (Glu1)-Fibrinopeptide B; H-Glu-Gly-Val-Asn-Asp-Asn-Glu-Glu-Gly-Phe-Phe-Ser-Ala-Arg-OH; EGVNDNEEGFFSAR-OH; Bachem H-2950 / 4016660; CAS 103213-49-6 | MS internal standard, lock mass, calibrant peptide, coagulation marker analog, acidic peptide, free acid, water-soluble, ESI-LC-MS/MS | Linear 14-aa acidic short peptide, sequence H-Glu-Gly-Val-Asn-Asp-Asn-Glu-Glu-Gly-Phe-Phe-Ser-Ala-Arg-OH (EGVNDNEEGFFSAR-OH); same sequence as human FpB (pEGVNDNEEGFFSAR) but with a free N-terminal Glu (free alpha-amino group) instead of the native pyroglutamate; C-terminal free acid (no amidation); side-chain net charge ~-3 (1 Arg positive, 1 Asp + 3 Glu negative, plus free N-terminus +1 and free C-terminus -1); contains two consecutive Phe (Phe10-Phe11) as the main hydrophobic residues, hydrophobic fraction ~29%; contains 2 Asn (deamidation-prone); no Cys/Met/Trp (no disulfide/oxidation/photo-oxidation); rich in acidic residues, low pI, good aqueous solubility; with a free N-terminal amino group and an exact, stable peptide ion outside typical proteolytic backgrounds, it is widely used as a lock-mass internal standard in ESI-LC-MS/MS (doubly charged ion m/z ~785.84) | C66H95N19O26 | 1569.67 | 1570.59 | Free acid (C-terminal -COOH); no counterion salt; powder | 14 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-EFPB-01 | White to off-white powder | Store at -20°C, sealed, desiccated, protected from light (~2 years). Aliquot to avoid repeated freeze-thaw. No Met/Cys/Trp-low oxidation/photo-oxidation; Asn may slowly deamidate on long storage. For MS use, aliquot at high concentration in small volumes to minimize repeated opening. | 2–8°C for up to ~1 month; working aliquots at -20°C; stable for shipping at ambient. | Acidic short peptide with good water solubility (soluble in aqueous, 50% ACN-water or 0.1% formic acid-compatible with MS mobile phases); the two Phe residues add slight hydrophobicity-use dilute acetic acid if needed. No Cys/Met/Trp. As an MS internal standard it is typically added at very low concentration (fmol–pmol/uL) or used as a lock-mass spray; the lyophilate is hygroscopic-weigh quickly and reseal. | solid stable for ~2 years at -20°C, sealed and desiccated; keep the desiccator sealed and the weighing environment dry. |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Pathways |
| PEP-EFPB-01 | The N-terminal free-Glu linear analog of human fibrinopeptide B-an exact-mass, stable-ion peptide widely used as a lock-mass internal standard/calibrant in ESI-LC-MS/MS, and for quantitative studies of coagulation-related peptides. | [Glu1]-fibrinopeptide B is a synthetic analog of human fibrinopeptide B (Bβ1-14): the native N-terminal pyroglutamate is replaced by a free L-glutamate (free N-terminal alpha-amino), sequence EGVNDNEEGFFSAR-OH. Unlike the pyroglutamylated form, the N-terminus is not cyclized, producing a stable, reproducibly protonated multiply-charged ion in electrospray ionization: the doubly charged [m+2H]2+ at m/z ~785.84 and singly charged at m/z ~1570.6. Because of its exact mass, strong signal and absence from typical enzymatic/biological backgrounds, it is recommended by Waters and Bachem as an external calibrant or on-line lock-mass internal standard for LC-ESI-MS/MS, used for mass-axis calibration, mass-drift correction and peak locking; it is also added as an internal reference in peptide mapping, proteomics and quantitative workflows for coagulation-related peptides. Biologically it retains the Bβ1-14 FpB scaffold, enabling studies of how N-terminal pyroglutamation affects fibrinopeptide stability, thrombin cleavage and circulating metabolism. This free-acid standard is suitable for MS quantitation and coagulation/fibrin research. | MS calibration/lock-mass internal standard, proteomics, coagulation & hemostasis, thrombosis research, peptide mapping, biomarker quantitative method development | ESI-MS lock-mass ion (doubly charged m/z ~785.84; singly charged m/z ~1570.6); fibrinogen Bβ-chain residues 1-14 scaffold |
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