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| Description | Biotin-KKKRKV acetate is the biotinylated acetate form of the classical SV40 large T-antigen NLS (KKKRKV). This basic hexapeptide is recognized by importin α/β to drive nuclear import; the biotin tag enables conjugation to nanoparticles, nucleic acids or protein cargoes for nuclear-targeted delivery and import-mechanism studies. |
| Peptide purity (HPLC) | ≥95% |
| Molecular formula | C58H113N21O16·xCH3COOH |
| Molecular weight (Da) | ~1129 (free biotinylated peptide) |
| Structure | Linear 6-mer (KKKRKV NLS, biotinylated acetate) |
| Solubility | Readily soluble in water/PBS |
| Storage | Powder, −20°C, dry, light-protected |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| Biotin-KKKRKV Acetate, N-terminally Biotin-Labeled SV40 Large T Antigen Nuclear Localization Signal (NLS) Hexapeptide | Biotin-KKKRKV; Biotin-SV40 NLS; biotinylated SV40 large T-antigen nuclear localization signal hexapeptide; N-terminally biotinylated NLS peptide; cell-penetrating / nuclear-targeting peptide | biotinylated peptide, nuclear localization signal (NLS), SV40 Large T antigen, cell-penetrating peptide, nuclear-targeted delivery, importin alpha/beta binding, acetate salt | Linear hexapeptide, sequence Lys-Lys-Lys-Arg-Lys-Val (KKKRKV)-the canonical SV40 large T-antigen nuclear localization signal (NLS); N-terminal alpha-amine forms an amide bond with biotin; free C-terminal carboxyl; 4 Lys + 1 Arg = +5 side-chain positive charges, net ~+4 (strongly basic); no Cys/Met/Trp/Tyr (no disulfide, no oxidation, no photo-oxidation); the classic monopartite NLS recognized by importin alpha (karyopherin alpha); acetate counterion | C45H85N15O9S | 1011.64 | 1012.33 | Acetate salt; N-terminal biotin label | 6 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-BIKKKRKV-01 | White to off-white powder | Store at -20°C, sealed and desiccated, ~2 years. The strongly basic peptide is hygroscopic-keep tightly sealed and dry; store in a desiccator. | 2-8°C for ~1 month; working aliquots at -20°C. | Strongly basic peptide (4 Lys + 1 Arg) with excellent water solubility (mg/mL grade in pure water/aqueous buffer; no DMSO needed). Biotinylation retains high aqueous solubility; can be coupled to streptavidin-coated carriers/nanoparticles for nuclear-targeted delivery. No Cys/Met/Trp-chemically stable and resistant to oxidation. The acetate form is suitable for cell and in-vivo experiments. | solid stable ~2 years at -20°C, sealed and desiccated; because of its strong basicity and hygroscopicity, weigh promptly after opening and reseal tightly; store in a desiccator. |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Pathways |
| PEP-BIKKKRKV-01 | N-terminally biotinylated SV40 large T-antigen canonical NLS hexapeptide KKKRKV, recognized by importin alpha/beta to drive nuclear import-used for nuclear-transport mechanism studies, cell-penetrating/nuclear-targeted delivery and streptavidin affinity purification. | KKKRKV is the most classic and best-studied classical nuclear localization signal (NLS) from simian virus 40 (SV40) large T antigen. In the cytoplasm, NLS-bearing cargo is recognized by importin alpha (karyopherin alpha) via its ARM-repeat domain, then bound by importin beta to engage the nuclear pore complex (NPC); directional transport is driven by the Ran-GTPase gradient. As a minimal functional NLS, KKKRKV can be fused to proteins, peptides, nucleic acids, liposomes or nanoparticles to route exogenous cargo to the nucleus-it is the most widely used peptide signal in nuclear-targeted delivery. N-terminal biotinylation enables, via streptavidin bridging: (1) conjugation of the NLS to streptavidin-coated nanoparticles/liposomes/fluorescent microspheres to build nuclear-targeted delivery systems; (2) use as an affinity pull-down probe for importin alpha/beta nuclear transport receptors to study nuclear-import mechanisms; (3) as a cell-penetrating peptide (CPP) to assist macromolecular cellular uptake and nuclear entry. This strongly basic peptide (+5 side-chain charge) is highly water-soluble and supplied as the acetate salt, suitable for cell and in-vivo work; note that NLS function depends on importin alpha recognition-excess free NLS will competitively inhibit endogenous nuclear import. | Nuclear transport (NLS) mechanism, importin alpha/beta nuclear import, SV40 large T antigen, nuclear-targeted delivery (drugs/genes/nanoparticles), cell-penetrating peptides (CPP), biotin-streptavidin affinity purification, macromolecular nuclear delivery | Importin alpha (karyopherin alpha, the NLS receptor) / importin beta heterodimer nuclear transport receptors; nuclear pore complex (NPC); high-affinity binding to streptavidin |
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