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| Description | This FITC-labeled probe mimics the Nrf2 ETGE/DLGE motif that binds the Keap1 Kelch domain. It is used in fluorescence-polarization or titration assays to quantify Keap1 affinity and as a competitive probe to screen small molecules that disrupt Keap1-Nrf2 and activate the endogenous antioxidant response. |
| Peptide purity (HPLC) | ≥95% |
| Molecular weight (Da) | ~1500–2200 (probe length, FITC conjugate) |
| Structure type | Linear Keap1-binding probe peptide (FITC-labeled) |
| Solubility | Readily soluble in water/PBS |
| Storage | Powder, −20°C, dry, light-protected |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| FITC-labelled Keap1-Nrf2 Probe (FITC-LDEETGEFL-NH2; Nrf2 Neh2 ETGE motif 9-mer) | FITC-Keap1-Nrf2 Probe; FITC-LDEETGEFL-NH2; FITC-Nrf2(76-84); KL9-FITC; FP tracer for Keap1 | fluorescently labelled peptide, FITC label, Keap1-Nrf2 probe, oxidative stress, Nrf2 pathway, fluorescence polarization (FP), light-sensitive | Linear 9-mer, sequence Leu-Asp-Glu-Glu-Thr-Gly-Glu-Phe-Leu (LDEETGEFL), corresponding to human Nrf2 Neh2-domain residues 76–84 (the canonical DxETGE Keap1-binding motif); the N-terminal alpha-amine forms a thiourea bond with FITC (fluorescein isothiocyanate); C-terminus is amidated (-NH2) for enhanced stability; contains the core ETGE (Thr-Gly-Glu) binding motif flanked by Asp/Glu acidic residues and Leu/Phe hydrophobic residues; net charge at pH 7.4 is approximately -3 to -4 (three Glu + one Asp); FITC is a green fluorophore (Ex/Em ~495/519 nm) specifically used in fluorescence polarization (FP) binding assays and competition screening against the Keap1 Kelch domain | C67H81N11O23S | 1439.52 | 1440.50 | Acetate / free base (C-terminal amide; FITC labelled; TFA salt available on request) | 9 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-KNFP-01 | Pale yellow to orange-yellow powder (FITC fluorescein colour) | Store at -20°C, sealed, desiccated, protected from light (amber vial or aluminium-foil wrap). Aliquot to avoid repeated freeze-thaw. FITC is light-sensitive-protect from light throughout. | 2–8°C, protected from light, for ~1–2 weeks; prepare working solutions fresh for FP assays and use within hours in black 96/384-well plates at room temperature in the dark. | Good water solubility (multi-acidic peptide, readily dissolves in water/neutral buffer). FITC requires strict light protection (amber vials/black plates/foil) to prevent photobleaching. C-terminal amidation increases proteolytic stability. No Met/Cys/Trp-low oxidation risk. Recommend buffer containing 0.01–0.05% Tween-20 in PBS to reduce non-specific adsorption; prepare fresh and avoid repeated freeze-thaw. | solid is stable for ~1 year at -20°C, sealed, desiccated and protected from light; avoid moisture and light. |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Pathways |
| PEP-KNFP-01 | N-terminal FITC-labelled Nrf2 ETGE-motif 9-mer (LDEETGEFL-NH2) that specifically binds the Keap1 Kelch domain; used in Keap1-Nrf2 PPI fluorescence-polarization (FP) assays and Nrf2-activator screening. | Nrf2 (NF-E2-related factor 2) is the master transcription factor of the cellular antioxidant response. Under homeostatic conditions it is captured and ubiquitinated for degradation by the cytoplasmic adaptor Keap1 (Kelch-like ECH-associated protein 1) through its Kelch-repeat domain. Upon oxidative stress or electrophilic modification of Keap1 cysteines, Nrf2 is released, translocates to the nucleus and drives antioxidant genes such as HO-1, NQO1 and GCLC. The Nrf2 Neh2 domain contains two Keap1-binding motifs: the high-affinity ETGE (residues 79–82) and the low-affinity DLG (residues 29–31). Extending ETGE into the 9-mer LDEETGEFL (Nrf2 residues 76–84) yields the minimal high-affinity Keap1-Kelch binder. This product is N-terminally conjugated to FITC (fluorescein isothiocyanate, Ex/Em ~495/519 nm) and C-terminally amidated; it is the standard FP tracer used in the literature and by vendors such as MCE/BPS. In a typical assay, a fixed concentration of FITC-LDEETGEFL is incubated with recombinant Keap1 Kelch protein (Keap1 318–609/324–609); binding slows tumbling and increases the polarization value. Addition of a competing small molecule or peptide reduces polarization, from which IC50/Ki can be quantified. Typical applications: (1) high-throughput screening of Keap1-Nrf2 protein-protein interaction (PPI) inhibitors; (2) in-vitro affinity profiling of natural-product/synthetic Nrf2 activators; (3) oxidative-stress, chemoprevention and anti-inflammatory drug discovery; (4) competitive-binding validation of optimized peptide inhibitors (cyclic/stapled peptides). Assays must use black low-binding plates, be protected from light and contain Tween-20 to minimize non-specific adsorption and ensure a robust FP signal window. | Keap1-Nrf2 pathway, oxidative stress and antioxidant response, protein-protein interaction (PPI) screening, fluorescence polarization (FP) HTS, Nrf2 activator / chemopreventive drug discovery, anti-inflammation and cytoprotection, cancer chemoprevention, peptide drug design | Keap1 Kelch-repeat domain (Keap1 318–609); Nrf2 Neh2 ETGE motif (residues 76–84, LDEETGEFL); used for competitive screening of Nrf2 activators |
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