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| Description | Three tandem DYKDDDDK (FLAG) repeats, greatly improving anti-FLAG detection and purification; a common N/C fusion tag for western blot, IP and affinity purification. |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure (Sequence) | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| 3x DYKDDDDK Tag (3×FLAG octapeptide tag, three directly tandem repeats, 24-mer) | 3x FLAG peptide; 3×DYKDDDDK; (DYKDDDDK)3; 3x FLAG-tag; CAS 921765-76-6 | tag peptide, FLAG epitope, affinity purification, immuno-detection, recombinant protein | Linear 24-mer, H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH; three copies of the classic FLAG octapeptide DYKDDDDK directly tandem-linked N→C with no linker residues; distinct from the canonical 22-aa 3×FLAG (DYKDHDGDYKDHDIDYKDDDDK, MW≈2862); contains Asp×15, Tyr×3, Lys×3; highly hydrophilic; no Cys/Met | C123H176N30O58 | 3001.17 | 3002.92 | Not specified (synthetic peptide is routinely supplied as TFA salt; final COA prevails); CAS 921765-76-6 | 24 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-3XFLAG-01 | White to off-white powder (hygroscopic if supplied as TFA salt) | Store at −20°C, sealed, desiccated, protected from light; aliquot to avoid repeated freeze-thaw; ~2 years. Contains Tyr×3 - keep dark. If supplied as TFA salt, keep in a desiccator. | 2–8°C, desiccated, dark, for ~2 weeks; working aliquots at −20°C, dark; ship on blue ice. | Extremely hydrophilic (15 Asp residues); excellent water solubility - directly dissolves in water or PBS at 1–5 mM without DMSO. Prepare fresh; stable for 1–2 weeks at 4°C dark. No Cys/Met, therefore no oxidation risk. For competitive elution from anti-FLAG resin, use 100–500 μg/mL (0.3–1.5 mM) at 4°C for mild elution. | solid stable ~2 years at −20°C, sealed, desiccated, dark. TFA-salt batches are hygroscopic - store in a desiccator under inert gas. Contains Tyr×3 - keep dark for long-term storage. No Cys/Met - no reducing agent required. |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Receptors |
| PEP-3XFLAG-01 | 3×FLAG epitope tag peptide: three directly tandem copies of the classic FLAG octapeptide DYKDDDDK (24-mer, CAS 921765-76-6). Very high Asp content, excellent water solubility, no Cys/Met. Used for anti-FLAG immuno-detection of recombinant proteins and for competitive peptide elution from FLAG affinity resin. Distinct from the canonical 22-aa 3×FLAG (DYKDHDGDYKDHDIDYKDDDDK, MW≈2862). | The FLAG epitope (DYKDDDDK) is a hydrophilic octapeptide fusion tag designed by Evan et al. in 1984. Because it is lowly immunogenic, absent from native mammalian proteins, and can be removed by enterokinase immediately adjacent to the N-terminus, it is one of the most widely used tags for recombinant-protein expression, detection, and purification. 3×FLAG - the 24-mer (DYKDDDDK)₃ direct tandem repeat popularized by Sigma/Millipore - shows markedly higher avidity and detection sensitivity toward anti-FLAG M1/M2/M5 monoclonal antibodies (e.g., anti-FLAG M2 affinity gel) than single-copy FLAG. It is used for: (1) Western blot, ELISA, immunofluorescence, and flow-cytometric detection of low-abundance or weakly expressed recombinant proteins; (2) one-step anti-FLAG M2 affinity purification followed by gentle competitive elution with free 3×FLAG peptide (100–500 μg/mL), avoiding protein-activity loss caused by pH 3–4 glycine elution; (3) co-immunoprecipitation and protein–protein interaction studies; (4) in vivo/in vitro reporter localization and quantitation. This product (CAS 921765-76-6) is locked to the 24-mer (DYKDDDDK)₃ direct-tandem sequence, formula C123H176N30O58, average MW ≈ 3002.92 Da. It must be clearly distinguished from the canonical 22-aa 3×FLAG sequence DYKDHDGDYKDHDIDYKDDDDK (MW ≈ 2862 Da, with Asp-His-Asp-Gly/Ile linkers), which has slightly different antibody-epitope presentation and elution behavior. This product contains 15 Asp residues, is acidic (low pI), highly water-soluble, and free of Cys/Met - no oxidation or dimerization risk. | recombinant protein expression, FLAG-tag immuno-detection (WB/IF/FC/ELISA), anti-FLAG affinity purification and competitive elution, co-immunoprecipitation, protein–protein interaction, reporter localization and quantitation | anti-FLAG M1/M2/M5 monoclonal antibodies; FLAG M2 affinity resin; enterokinase (if tag removal is required) |
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