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| Description | OVA323-339 (ISQAVHAAHAEINEAGR, I-Ab-restricted CD4 helper epitope) conjugated to Cy3; used to track antigen presentation, uptake/localization and T-cell-antigen interactions by fluorescence. |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| Cy3-Ova (323-339) (Cy3 fluorescently labeled ovalbumin MHC-II CD4 Th epitope peptide; unlabeled OVA 323-339: CAS 92915-79-2) | Cy3-ISQAVHAAHAEINEAGR; Cy3-OVA(323-339); OVA 323-339 I-Ad Th epitope, Cy3-labeled; Cyanine3-labeled ovalbumin CD4 epitope; PEP-CY3OVA-01 | Cy3-labeled peptide, MHC-II epitope peptide, OVA(323-339), I-Ad restricted, CD4 Th epitope, DO11.10 hybridoma, OT-II transgenic T cells, antigen uptake & presentation tracing, light-protected | Cy3-labeled linear 17-mer, Cy3-Ile-Ser-Gln-Ala-Val-His-Ala-Ala-His-Ala-Glu-Ile-Asn-Glu-Ala-Gly-Arg-OH (Cy3-ISQAVHAAHAEINEAGR), derived from chicken ovalbumin (UniProt P01012) residues 323-339 (core nonamer QAVHAAHAE); Cy3 is a sulfated Cyanine3 fluorophore (bearing two sulfonate groups) coupled to the N-terminal alpha-amino group via an amide bond; lambda ex approx. 555 nm / lambda em approx. 570 nm, epsilon approx. 150,000 L mol-1 cm-1. | C105H156N28O32S2 | 2385.09 | 2386.69 | Commercial Cy3-labeled peptide (TFA/acetate counterion per COA) | 17 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-CY3OVA-01 | Deep red to purplish-red powder (characteristic Cyanine3 color). | Store at -20°C, sealed, desiccated and PROTECTED FROM LIGHT at all times (amber vial/aluminum foil wrap), approx. 2 years; aliquot into single-use portions to avoid repeated freeze-thaw. The Cy3 fluorophore must be kept in the dark at all times to prevent photooxidation and fluorescence quenching. | 2-8°C, light-protected, for approx. 2 weeks; working aliquots at -80°C, light-protected; avoid repeated freeze-thaw; prepare fresh as needed. | Good water solubility (amphipathic Th-epitope peptide); reconstitute directly in sterile water or pH 7.4 PBS/HBSS; prepare a 1-10 mg/mL stock, filter-sterilize through 0.22 µm and aliquot in the dark. Reconstituted solution stable approx. 1 week at 4°C or approx. 6 months at -80°C in light-protected aliquots, avoiding repeated freeze-thaw. Cy3 channel lambda ex approx. 555 nm / lambda em approx. 570 nm-keep wrapped in aluminum foil at all times; for live-cell tracing of dendritic-cell/macrophage antigen uptake, intracellular trafficking and MHC-II presentation, avoid strong illumination before detection. | solid stable approx. 2 years at -20°C, sealed, desiccated and light-protected; no Trp/Cys/Met, so the peptide backbone is chemically stable, but Cy3 is light-sensitive-weigh promptly after opening, reseal (wrap in foil) and avoid prolonged exposure to bright room light. |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Pathways |
| PEP-CY3OVA-01 | N-terminally sulfated-Cy3-labeled classic ovalbumin MHC-II I-A^d-restricted CD4 Th epitope (ISQAVHAAHAEINEAGR, 17-mer), for DO11.10/OT-II OVA-specific CD4 T-cell activation and live-cell fluorescence tracing of dendritic-cell antigen uptake and MHC-II presentation pathways. | Ovalbumin (OVA) is the major chicken egg-white protein; residues 323-339 (core nonamer QAVHAAHAE) constitute the most widely used CD4+ helper T-cell epitope presented by the mouse MHC-II molecule I-A^d (H-2^d haplotype, BALB/c mice) and serves as the model epitope for antigen-specific CD4 T-cell research; tool cells recognizing it include the DO11.10 OVA-specific T-cell hybridoma and OT-II transgenic CD4 T cells. This product is the N-terminally sulfated-Cy3 (Cyanine3) conjugate of OVA(323-339) (lambda ex approx. 555 nm / lambda em approx. 570 nm; orange-red/far-red channel with low spectral overlap with FITC/GFP). The Cy3 label enables real-time tracing of dendritic-cell (DC) and macrophage endocytic uptake of the peptide, intracellular trafficking (endosomal/lysosomal route) and loading onto MHC-II molecules, and supports fluorescence microscopy and flow-cytometric measurement of antigen loading on APCs, immunological synapse formation between T cells and APCs, and comparison of antigen-presentation efficiency. Because of the fluorophore, all handling and storage must be light-protected; unlabeled OVA(323-339) (CAS 92915-79-2) is the matched non-fluorescent control peptide of the same epitope. | Antigen presentation, MHC-II epitope, CD4 Th-cell activation, DO11.10 hybridoma / OT-II transgenic T cells, dendritic-cell uptake tracing, immunological synapse, fluorescence flow cytometry, vaccine model epitope | Mouse MHC-II molecule I-A^d (H-2^d); DO11.10 TCR / OT-II TCR; Cy3 fluorescence channel lambda ex approx. 555 nm / lambda em approx. 570 nm |
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