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| Description | A V5 tag with a free N-terminal cysteine for thiol-directed coupling to carriers, beads or fluorophores; used for immobilization and immunogen preparation. |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| Cysteine-added V5 epitope tag | Cys-V5 peptide; N-terminal Cys V5 tag peptide; CGKPIPNPLLGLDST; V5 epitope peptide (with coupling Cys); SV5 P/V protein epitope; PEP-CYSV5-01 | tag peptide, V5 epitope, N-terminal Cys coupling site, affinity purification, competitive elution, immuno-detection, oxidation-sensitive, TFA salt, CAS reference standard | Linear 15-mer, H-Cys-Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-OH (CGKPIPNPLLGLDST); a single cysteine is appended to the N-terminus of the classic V5 epitope GKPIPNPLLGLDST (the C-terminal 14-mer of simian virus 5, SV5, P/V protein) to provide a thiol-directed coupling site (for site-directed conjugation to KLH/BSA carrier proteins or beads/resins); contains Pro×3, Leu×3, Ile×1 (moderately hydrophobic) and Cys×1 (thiol, prone to oxidative dimerization). | C67H113N17O21S | 1523.8 | 1524.8 | Free-peptide formula/MW (registered form of CAS 922704-46-9; MCE HY-P5969 registers the free form C67H113N17O21S, MW 1524.8); synthetic peptides are commonly supplied as TFA salts ·C2HF3O2 (MW +113.99/+114.02, i.e. ~1638.8 as TFA salt) - actual salt form per COA | 15 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-CYSV5-01 | White to off-white solid (if supplied as TFA salt it is hygroscopic - reseal immediately, keep in a desiccator and weigh rapidly). | Store at −20°C, sealed, desiccated; aliquot to avoid repeated freeze-thaw; ~2 years. ⚠️ Contains an N-terminal free Cys (thiol) - must be sealed under Ar/N2 to exclude oxygen, kept away from oxidants and opened as infrequently as possible, to prevent Cys oxidation and disulfide dimerization. No Tyr/Trp - no light protection needed. If TFA salt - keep in a desiccator. | 2–8°C, desiccated, under inert gas, for ~2 weeks; prepare working solutions fresh and store aliquots at −20°C; ship on blue ice, sealed against oxidation. | Moderately hydrophilic-to-balanced solubility (the V5 epitope contains Pro×3/Leu×3/Ile×1, ~40% hydrophobic residues): first dissolve in a small amount of DMSO (~1–2 μL/mg) and then dilute with PBS pH 7.4 or water to 1–2 mg/mL (~0.7–1.3 mM), filtering through 0.22 μm. ⚠️ Contains free Cys - do not store solutions long-term: prepare fresh; if storage is needed, add 1–5 mM EDTA to chelate trace metals, flush with argon, and store aliquots at −20°C for ~1–2 weeks; keep away from oxidants and heavy metals (Cu2+). For competitive elution from anti-V5 resin, dilute in tag-free buffer to 1–5 mg/mL and elute mildly at 4°C. | solid stable ~2 years at −20°C, sealed, desiccated, under inert gas. Contains free Cys - oxygen exclusion / Ar-N2 flushing retards oxidative dimerization. If TFA salt - store in a desiccator. No Tyr/Trp - no light protection needed. |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Receptors |
| PEP-CYSV5-01 | A 15-mer V5 epitope peptide with an N-terminal cysteine (CGKPIPNPLLGLDST), CAS 922704-46-9 (MCE HY-P5969), MW 1523.80/1524.80; the N-terminal Cys provides a thiol-directed coupling site and the V5 epitope portion is recognized by anti-V5 mAbs (SV5-Pk1/V5-10) - used for V5 fusion protein detection, competitive elution, and site-directed conjugation to carriers/beads for immunogen preparation. | The V5 epitope (GKPIPNPLLGLDST) is derived from the C-terminal 14 residues of the P/V protein of simian virus 5 (SV5) and is one of the most widely used N/C-terminal tags in recombinant protein research, specifically recognized by anti-V5 monoclonal antibodies (clones SV5-Pk1, V5-10, etc.). This product appends one cysteine to the N-terminus of the epitope (CGKPIPNPLLGLDST, 15 residues in total) to provide a unique free thiol: (1) site-directed conjugation of the peptide to KLH/BSA/ovalbumin carrier proteins or to beads/resins via thiol chemistry (maleimide/SPDP), yielding immunogens or affinity ligands with uniform orientation that does not disrupt the V5 epitope; (2) use as a competing/blocking peptide for anti-V5 antibodies. CAS 922704-46-9 is registered by MedChemExpress (HY-P5969) as the Cys-V5 peptide (C67H113N17O21S, MW 1524.8), and ChemicalBook (MFCD03458947) registers the same formula and MW under the name "V5". Compared with the canonical Cys-free V5 peptide GKPIPNPLLGLDST (Proteintech V5-peptide, MW ≈1422), this product differs by exactly one dehydrated Cys residue (+103.14) - the three sources mutually confirm that CAS 922704-46-9 is the N-terminal-Cys V5 peptide. The formula and MW given here (C67H113N17O21S, exact 1523.80 / average 1524.80 Da) match the registration. Caveats: (1) the free Cys readily oxidizes to the dimer - store sealed under inert gas and prepare solutions fresh; (2) the V5 epitope is Pro/Leu-rich, so a small amount of DMSO is needed to assist dissolution; (3) the actual delivered salt form (free / TFA) is per COA - the TFA-salt MW is ~1638.8. | recombinant protein expression, V5-tag immuno-detection (WB/IP/IF/FC), anti-V5 affinity purification and competitive elution, thiol-directed immunogen conjugation, CAS reference standard | anti-V5 monoclonal antibodies (SV5-Pk1, V5-10); V5 affinity resin (V5-Trap); thiol-conjugation targets (maleimide/SPDP) |
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