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| Description | An 11-histidine tag (TFA salt) with higher metal-chelate affinity than His6, for one-step purification of low-abundance recombinant proteins. |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| His11 Peptide, TFA salt (11-mer HHHHHHHHHHH; 即11×His标签肽三氟乙酸盐) | His11 TFA; 11xHis tag peptide (trifluoroacetate salt); polyhistidine peptide; HHHHHHHHHHH; H11; PEP-HIS11T-01 | tag peptide, polyhistidine tag, 11xHis, Ni-column competitive elution, affinity purification, highly hydrophilic, TFA salt, hygroscopic | Linear 11-mer, H-His-His-His-His-His-His-His-His-His-His-His-OH (HHHHHHHHHHH); eleven consecutive histidines, i.e. an 11×His tag; the imidazole side chains (pKa ≈6) are positively charged at neutral pH and chelate immobilized Ni2+/Co2+/Cu2+ metal ions (IMAC); trifluoroacetate (TFA) salt form. | C66H79N33O12 | 1525.66 | 1526.57 | TFA salt: free peptide C66H79N33O12 (MW 1525.66/1526.57) combined with trifluoroacetate ·C2HF3O2 (MW +113.99/+114.02), i.e. ~1639.65/1640.59 as the TFA salt; strongly hygroscopic - reseal immediately on opening, keep in a desiccator, weigh rapidly; no CAS registered | 11 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-HIS11T-01 | White to off-white solid (TFA salt, strongly hygroscopic - store sealed in a desiccator and weigh rapidly). | Store at −20°C, sealed and dry (the TFA salt is strongly hygroscopic - keep in a desiccator / sealed under Ar-N2); aliquot to avoid repeated freeze-thaw; ~2 years. No Met/Cys/Trp/Tyr, so no special light/oxidation protection needed. ⚠️ Do not store long-term in buffers containing Ni2+/Co2+ metal ions (His chelates metal ions). | 2–8°C, sealed in a desiccator, for ~2 weeks; working aliquots at −20°C; ship on blue ice, moisture-protected. | Highly hydrophilic polyhistidine peptide - very soluble in water or neutral buffers (PBS, Tris-HCl); directly prepare 2–5 mg/mL (~1.3–3.3 mM) stock without DMSO. For competitive elution of His-tagged fusion proteins from Ni-NTA/Co columns, a 20–250 mM imidazole gradient is conventional; as an 11×His peptide itself, this product can directly compete for elution in tag-free buffer (working concentration 1–5 mg/mL), yielding eluate free of antibody contamination. ⚠️ Avoid long-term contact with buffers carrying residual Ni2+ from column matrices; use at 4°C within ~1 week, aliquot and store at −20°C for 1–3 months; never repeatedly freeze-thaw. | TFA salt stable ~2 years at −20°C, sealed in a desiccator. Strongly hygroscopic - protect from moisture. No free Cys (no disulfide). No Met/Tyr/Trp - no oxidation/light protection needed. |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Receptors |
| PEP-HIS11T-01 | The trifluoroacetate salt of the 11×His tag peptide, 11-mer (HHHHHHHHHHH) (free peptide MW 1525.66/1526.57; TFA salt ~1639.65/1640.59); used for competitive elution of His fusion proteins from Ni-NTA/Co affinity resin and as an anti-His detection control; strongly hygroscopic - store in a desiccator. | The polyhistidine tag (His-tag) is the most widely used affinity tag in recombinant protein purification: consecutive imidazole side chains of 6–10+ histidines chelate immobilized Ni2+ (Ni-NTA), Co2+, Cu2+ or Zn2+ via coordination bonds at neutral pH (IMAC, immobilized metal-affinity chromatography), and are eluted competitively with imidazole. This product is eleven consecutive histidines (HHHHHHHHHHH, 11×His), which binds metal ions more tightly than the common 6×His tag. Applications: (1) as a free competing peptide for mild elution of His/11×His fusion proteins from Ni-NTA/Co columns or anti-His affinity resin (1–5 mg/mL in tag-free buffer), yielding eluate free of antibody heavy/light chain contamination and imidazole interference; (2) specificity blocking/competition control in WB/ELISA with anti-His mAbs (e.g. clones His-1, AD1.1.1); (3) IMAC methodology research and tag reference standards. This product is supplied as the trifluoroacetate salt (synthetic peptides conventionally carry TFA counterions after reversed-phase HPLC purification): the free peptide formula is C66H79N33O12, exact 1525.66 / average 1526.57 Da; with one TFA (C2HF3O2, +113.99/+114.02) the TFA-salt MW is ~1639.65/1640.59 Da. Note: the TFA salt is extremely hygroscopic - reseal immediately on opening, keep in a desiccator and weigh rapidly; avoid long-term contact of solutions with Ni2+/Co2+-containing buffers (His chelates metal ions, causing metal precipitation and peptide loss). It is the same sequence as the free-form version (PEP-HIS11-01, CAS 620927-23-3) but in a different salt form. | recombinant protein purification, Ni-NTA/Co IMAC affinity chromatography, His-tag competitive elution, anti-His immuno-detection control, polyhistidine tag methodology, tag reference standard | immobilized Ni-NTA/Co2+/Cu2+ metal ions (IMAC); anti-His monoclonal antibodies (His-1, AD1.1.1); His-tagged fusion proteins |
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