CAS 221216-83-7 EEepitope

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CAS 221216-83-7 EEepitope
Details
Glu-Glu (EE) tag/epitope peptide;
Category: Recombinant-protein epitope tag / immuno-detection tag.
Peptide Purity (HPLC) ≥95%
CAS No. 221216-83-7
Structure Acidic tag peptide (EE/Glu-Glu epitope)
Solubility Water, dilute ammonia
Storage Powder, −20°C, dry, light-protected
Category
Epitope Peptides
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Description

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Description The acidic EE tag derived from the HCMV immediate-early protein, recognized by anti-EE mAbs (e.g. 13E5); fused to recombinant proteins for Western blot, immunoprecipitation and localization.

 

Product Basic Information Table

 

Product Standard English Name Product Abbreviation / Alias Function Tags (comma-separated) Core Structure Molecular Formula Exact Mass (Da) Average MW (Da) Salt Form Length (aa)
EE Epitope EE Tag Peptide; Glu-Glu Epitope; EYMPME Epitope tag, Recombinant protein detection, Antibody purification EYMPME (H-Glu-Tyr-Met-Pro-Met-Glu-OH, linear 6-mer) C34H50N6O12S2 798.29 798.92 Free acid (per published formula) 6

 

Physicochemical Solubility & Storage Parameters

 

Product Code Appearance Long-term Storage Short-term Storage Solution Stability Solid Powder Stability
PEP-EE-01 White to off-white powder Store powder at ≤ -20°C, protected from light and moisture (TargetMol: 3 years at -20°C) Store sealed and dry at 4°C for use within 2-4 weeks; aliquot reconstituted solution and store at -20°C Freely soluble in aqueous buffers (2 acidic Glu residues, net charge -2); reconstitute to 1-5 mg/mL in PBS or Tris (pH 7.4); reconstituted solution stable for 1 year at -80°C (TargetMol); avoid repeated freeze-thaw; contains 2 Met - degas and avoid oxidants for long-term storage in solution powder stable for 3 years at -20°C, protected from light and moisture; N-terminal Glu may slowly cyclize to pyroglutamate (pGlu) over long storage (known variant)

 

Functional Description

 

Product Code Frontend Short Summary Detailed Biological Function Description Applicable Research Areas (comma-separated) Targets / Pathways
PEP-EE-01 EE epitope tag peptide (EYMPME, 6 aa), fragment of mouse polyomavirus middle T antigen (residues 314-319), specifically recognized by the anti-EE monoclonal antibody; used for detection, localization and purification of recombinant proteins The EE (Glu-Glu) epitope is a classic epitope-tag system: after fusing EYMPME to a recombinant protein, commercial anti-EE monoclonal antibodies enable expression validation and subcellular localization by Western blot, immunofluorescence and immunoprecipitation, and support immunoaffinity purification. The peptide itself can serve as a positive control, an immunogen for antibody production, or a competitor peptide in ELISA. Not related to the HIV gp41 epitope ELDKWA. Recombinant protein expression validation, Western blot, Immunofluorescence, Co-IP, Affinity purification, Antibody development, ELISA Anti-EE (anti-Glu-Glu) monoclonal antibody; no natural receptor

 

 

 

 

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