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| Description | The FLAG sequence DYKDDDDK with an azido-PEG3 spacer for click chemistry (CuAAC/SPAAC) conjugation to fluorophores, biotin or carriers; used for site-specific labeling, detection and purification of FLAG-fusion proteins. |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| Azido-PEG3-DYKDDDDK (azido-PEG3-propionyl-FLAG tag octamer) | Azido-PEG3-DYKDDDDK; Azido-PEG3-FLAG; N3-PEG3-COOH-FLAG; Bachem 4099640/H-7924; CAS 2243206-95-1 | click chemistry, azide (N3) group, SPAAC/CuAAC cycloaddition, PEG3 flexible spacer, FLAG tag (DYKDDDDK), anti-FLAG detection/purification, bioconjugation, light-sensitive | Azido-PEG3-propionyl-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH (N3-PEG3-CO-DYKDDDDK-OH, 8-mer). The N-terminal alpha-amine of the FLAG peptide DYKDDDDK is linked via an amide bond to an azido-PEG3-propionyl handle (N3-CH2CH2-(OCH2CH2)3-CH2CH2-CO-; the acid form C9H17N3O5 loses H2O on amide coupling). The FLAG core DYKDDDDK carries five Asp and two Lys and is highly hydrophilic; the terminal azide is the click-chemistry handle for CuAAC (alkyne) or SPAAC (cyclooctyne/DBCO); free C-terminal carboxyl. | C50H75N13O24 | 1241.50 | 1242.22 | TFA salt (trifluoroacetate, as noted by Bachem); published formula/MW are for the TFA-free active peptide moiety C50H75N13O24 | 8 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-AZFLAG-01 | White to off-white powder (Bachem #4099640, old H-7924; CAS 2243206-95-1) | Store below -15 C (preferably -20 C), sealed, dry and protected from light (amber tube/foil) to avoid azide photolysis; aliquot to avoid freeze-thaw | Short-term at 2-8 C in the dark; working aliquots at -20 C protected from light, freshly prepared preferred; avoid repeated freeze-thaw; ship cold-chain in the dark | The FLAG sequence (five acidic Asp) is strongly hydrophilic and water-soluble; dissolves in water or neutral buffer (PBS, HEPES). Contains an azide: protect from light (photosensitive), avoid reducing agents (azide can be reduced and lose reactivity), and keep away from alkyne/cyclooctyne (DBCO) reagents before use to prevent premature click reaction; the TFA salt is hygroscopic, weigh dry. After click coupling, the product is detected by anti-FLAG (M2) antibody via ELISA / Western blot / immunoaffinity purification. | solid stable below -15 C, sealed, dry and light-protected; the azide is light-sensitive and the TFA salt is hygroscopic-maintain light protection, dryness and minimal air exposure; do not contact reducing agents |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Pathways |
| PEP-AZFLAG-01 | An N-terminally azido-PEG3-spacer-modified FLAG tag peptide DYKDDDDK (CAS 2243206-95-1, Bachem); the azide is used to click the FLAG tag onto alkyne/DBCO molecules, after which the construct is detected or affinity-purified with anti-FLAG antibody. | Azido-PEG3-DYKDDDDK (Bachem #4099640, old H-7924; CAS 2243206-95-1) has three components: (1) the C-terminal classic FLAG epitope DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), specifically recognized by anti-FLAG monoclonal antibodies (e.g., M2), enabling convenient detection, quantitation and purification by ELISA, Western blot or anti-FLAG affinity chromatography; (2) an N-terminal azido-PEG3-propionyl flexible spacer attached to the peptide's alpha-amine by an amide bond; (3) a terminal azide (N3), the click-chemistry handle. The azide undergoes efficient, bioorthogonal cycloaddition with alkynes (CuAAC, Cu(I)-catalyzed) or cyclooctynes/DBCO (copper-free SPAAC) functionalized proteins, nucleic acids, small molecules or material surfaces, site-specifically attaching the FLAG tag. The PEG3 spacer improves solubility and reduces steric interference between the FLAG epitope and the conjugated molecule. Typical uses: FLAG-labeling of alkyne/DBCO-functionalized or metabolically azide-labeled cell-surface molecules for anti-FLAG enrichment and detection; construction of FLAG-tagged probes; and service as a detectable/capturable linker in bioorthogonal chemistry, activity-based target profiling, surface immobilization and immunoaffinity purification. Keep the azide light-protected and away from reductants, and do not mix with alkyne reagents before the intended coupling. | CuAAC/SPAAC bioconjugation, FLAG-tag detection/affinity purification, bioorthogonal labeling, site-specific coupling to alkyne/DBCO molecules, cell-surface protein labeling, immuno-enrichment / Western blot | Azide-alkyne cycloaddition (CuAAC/SPAAC); anti-FLAG (M2) antibody; alkyne/cyclooctyne (DBCO)-functionalized molecules |
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