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| Description | A de novo-designed 9-aa tag binding the NbALFA nanobody with high affinity and reversibly; used for ultrasensitive detection, live-cell imaging, IP and purification. |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| ALFA-tag Peptide (SRLEEELRRRLTE) | ALFA tag, CAS 2413353-86-1, NanoTag universal epitope tag (NbALFA-binding peptide) | Epitope tag, ALFA-tag, NbALFA nanobody, protein purification, live-cell imaging, universal tag | H-Ser-Arg-Leu-Glu-Glu-Glu-Leu-Arg-Arg-Arg-Leu-Thr-Glu-OH (linear 13-mer, free N-terminal H and C-terminal OH; de-novo designed sequence; no Cys/Met/Trp/Tyr) | C69H123N25O24 | 1685.92 | 1686.89 | TFA salt (typical) | 13 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-ALFAT-01 | White to off-white powder (TFA salt is hygroscopic - reseal immediately after opening) | Store sealed, dry and protected from light at -20°C; aliquot into single-use portions and avoid repeated freeze-thaw; stable for ~2 years; keep TFA salt in a desiccator | Store sealed, dry and protected from light at 4°C, use within 2-4 weeks; after reconstitution, aliquot and store below -20°C | Highly hydrophilic (4 Arg, 4 Glu; near-neutral net charge at physiological pH); directly soluble in water or PBS (pH 7.4) at 1-2 mg/mL without DMSO; prepare fresh, stable ~1 week at 4°C; handle under routine light protection | powder stable for ~2 years below -20°C when sealed, dry and protected from light; TFA salt is hygroscopic - store in a desiccator |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Pathways |
| PEP-ALFAT-01 | A de-novo designed 13-aa universal epitope tag (SRLEEELRRRLTE, CAS 2413353-86-1) recognized by the high-affinity NbALFA nanobody (~26 pM); used for recombinant-protein detection, affinity purification and live-cell / super-resolution imaging. | The ALFA-tag is a 13-aa peptide tag de-novo designed by Götzke et al. (2019, J. Mol. Biol.; NanoTag Biotechnologies), sequence SRLEEELRRRLTE (Ser-Arg-Leu-Glu-Glu-Glu-Leu-Arg-Arg-Arg-Leu-Thr-Glu). It forms a stable monomeric alpha-helix in solution, carries a near-neutral charge at physiological pH, has minimal impact on fused-protein folding, and is absent from common model organisms. Its cognate nanobody, NbALFA, binds the ALFA tag with an extremely high affinity of ~26 pM; paired with ALFA Selector affinity resin it enables mild, reproducible one-step purification, and fluorescently labeled NbALFA is used in Western blotting, immunofluorescence and live-cell super-resolution (e.g. STED) imaging. This synthetic ALFA-tag peptide serves as a competitor peptide, a binding-affinity control for anti-ALFA antibodies, and a reagent for studying NbALFA epitope recognition. Source: de-novo designed sequence (Götzke et al. 2019). | Protein engineering, epitope-tag systems, nanobody applications, recombinant-protein purification, live-cell imaging, super-resolution microscopy | NbALFA anti-ALFA nanobody; ALFA Selector affinity resin |
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