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| Description | An 11-histidine long tag binding Ni/Co chelate resins with high affinity, for efficient purification and detection of low-abundance proteins. |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| Undecahistidine tag | His11; 11xHis tag peptide; polyhistidine peptide; HHHHHHHHHHH; H11; CAS 620927-23-3; PEP-HIS11-01 | tag peptide, polyhistidine tag, 11xHis, Ni-column competitive elution, affinity purification, highly hydrophilic, CAS reference standard | Linear 11-mer, H-His-His-His-His-His-His-His-His-His-His-His-OH (HHHHHHHHHHH); eleven consecutive histidines, i.e. an 11×His tag; imidazole side chains (pKa ≈6) are positively charged at neutral pH and chelate immobilized Ni2+/Co2+/Cu2+ metal ions (IMAC); same sequence as the TFA-salt counterpart in this batch (#9, PEP-HIS11T-01) but a different salt form. | C66H79N33O12 | 1525.66 | 1526.57 | Free peptide (registered form of CAS 620927-23-3; MCE/Fisher register C66H79N33O12, MW 1526.55); no TFA annotation in catalog; synthetic peptides are commonly supplied as TFA salts ·C2HF3O2 (MW +113.99/+114.02) - actual salt form per COA | 11 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-HIS11-01 | White to off-white solid (salt form not annotated; if supplied as TFA salt it is hygroscopic - reseal immediately and keep in a desiccator). | Store at −20°C, sealed and dry; aliquot to avoid repeated freeze-thaw; ~2 years. No Met/Cys/Trp/Tyr, so no special light/oxidation protection needed. ⚠️ Do not store long-term in buffers containing Ni2+/Co2+ metal ions. If TFA salt - keep in a desiccator. | 2–8°C, sealed and dry, for ~2 weeks; working aliquots at −20°C; ship on blue ice. | Highly hydrophilic polyhistidine peptide - very soluble in water or neutral buffers (PBS, Tris-HCl); directly prepare 2–5 mg/mL (~1.3–3.3 mM) stock without DMSO. For competitive elution of His fusion proteins from Ni-NTA/Co columns, this peptide can directly compete in tag-free buffer (working concentration 1–5 mg/mL), yielding eluate free of antibody contamination. ⚠️ Avoid long-term contact with buffers carrying residual Ni2+ from column matrices; use at 4°C within ~1 week, aliquot and store at −20°C for 1–3 months; never repeatedly freeze-thaw. | solid stable ~2 years at −20°C, sealed and dry. No free Cys (no disulfide). No Met/Tyr/Trp - no oxidation/light protection needed. If TFA salt - store in a desiccator. |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Receptors |
| PEP-HIS11-01 | The 11×His tag peptide, 11-mer (HHHHHHHHHHH), registered under CAS 620927-23-3 (C66H79N33O12, MW 1525.66/1526.57); used for competitive elution of His fusion proteins from Ni-NTA/Co affinity resin and as an anti-His detection control; same sequence as the TFA-salt counterpart in this batch (#9) but a different salt form. | The polyhistidine tag (His-tag) exploits consecutive histidine imidazole side chains to chelate immobilized Ni2+ (Ni-NTA), Co2+, Cu2+ and Zn2+ at neutral pH (IMAC), and is the gold-standard tag for recombinant protein purification. This product is eleven consecutive histidines (HHHHHHHHHHH, 11×His), with higher metal affinity than the common 6×His tag. CAS 620927-23-3 is consistently registered by MedChemExpress/Fisher Scientific and ChemicalBook as this exact 11-mer (C66H79N33O12, MW ≈1526.55, sequence HHHHHHHHHHH), in agreement with the formula and MW given here. Main applications: (1) as a free competing peptide for mild elution of His/11×His fusion proteins from Ni-NTA/Co columns or anti-His affinity resin (1–5 mg/mL in tag-free buffer), yielding eluate free of antibody contamination and imidazole residue; (2) specificity blocking/competition control in WB/ELISA with anti-His mAbs (His-1, AD1.1.1, etc.); (3) IMAC methodology research and CAS reference material. This product is presented in the CAS-registered free form: formula C66H79N33O12, exact 1525.66 / average 1526.57 Da. It is the exact same sequence as the TFA-salt version in this batch (#9, PEP-HIS11T-01; TFA-salt MW ~1639.65/1640.59) - differing only in salt form. Note: avoid long-term contact of solutions with Ni2+/Co2+-containing buffers (His chelates metal ions); the actual delivered salt form (free base / TFA) is per COA. | recombinant protein purification, Ni-NTA/Co IMAC affinity chromatography, His-tag competitive elution, anti-His immuno-detection control, polyhistidine tag methodology, CAS reference standard | immobilized Ni-NTA/Co2+/Cu2+ metal ions (IMAC); anti-His monoclonal antibodies (His-1, AD1.1.1); His-tagged fusion proteins |
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