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| Description | Fibronectin Active Fragment Control (CAS: 97461-84-2) is the control sequence of the fibronectin active peptide fragment, sequence Gly-Arg-Gly-Glu-Ser (GRGES), molecular formula C₁₈H₃₂N₈O₉, MW 504.49 Da. Compared with the RGD active sequence, this peptide replaces aspartic acid (Asp) with glutamic acid (Glu), losing integrin-mediated adhesion activity; it is the standard negative-control peptide for cell-adhesion, integrin-binding, and RGD-function validation assays. |
| Structure type | Linear 5-mer (GRGES negative-control variant) |
| Solubility | Readily soluble in water, PBS |
| Storage | Powder -20°C, dry, protected from light |
Product Basic Information Table
| Product Standard English Name | Product Abbreviation / Alias | Function Tags (comma-separated) | Core Structure | Molecular Formula | Exact Mass (Da) | Average MW (Da) | Salt Form | Length (aa) |
| GRGES (Gly-Arg-Gly-Glu-Ser, inactive negative-control peptide of the fibronectin RGD active fragment) | GRGES; Gly-Arg-Gly-Glu-Ser; G-R-G-E-S; Fibronectin inactive control peptide; negative control for GRGDS; RGE control peptide; inactive control for GRGDSP/GRGDS; CAS 97461-84-2; free acid | cell-adhesion negative control, RGD control peptide, fibronectin fragment, inactive control peptide, integrin-binding control, assay control, free acid, water-soluble, chemically stable | Linear 5-aa short control peptide, sequence Gly-Arg-Gly-Glu-Ser (G-R-G-E-S); a site-directed inactive variant of active GRGDS (Gly-Arg-Gly-Asp-Ser)-the Asp(D) critical for integrin binding within the RGD core is replaced by Glu(E) (D→E), adding one side-chain methylene (-CH2-); side-chain net charge ~0 (Arg +1, Glu -1; ~0 including N-terminus +1 and C-terminus -1); no Cys, no Met, no Trp; nearly identical in length, physicochemistry and charge to GRGDS, yet loses RGD-dependent integrin binding and pro-adhesive activity because the key carboxylate side-chain length/geometry is altered; supplied as the free acid; CAS 97461-84-2 | C18H32N8O9 | 504.23 | 504.50 | Free acid; no counterion; CAS 97461-84-2 | 5 |
Physicochemical Solubility & Storage Parameters
| Product Code | Appearance | Long-term Storage | Short-term Storage | Solution Stability | Solid Powder Stability |
| PEP-FNAC-01 | White to off-white powder | Store at -20°C, sealed, desiccated, protected from light (~2 years). Aliquot to avoid repeated freeze-thaw. No Cys/Met/Trp-low oxidation/photo-oxidation risk; the short peptide is chemically stable. | 2–8°C for up to ~1 month; working aliquots at -20°C; stable for shipping at ambient. | A short peptide with physicochemical properties highly similar to GRGDS; good water solubility-dissolves directly in water or standard aqueous buffers (e.g., PBS) to several mg/mL, chemically stable and non-aggregating. No Cys (no disulfide), no Met (no oxidation), no Trp (no photo-oxidation). Use at the same concentration, solvent and pH as the active GRGDS/GRGDSP paired control; add directly to culture medium for competitive adhesion/migration assays, or coat substrates at the same density as the RGD peptide. | solid stable for ~2 years at -20°C, sealed and desiccated; not strongly hygroscopic-keep in a desiccator. |
Functional Description
| Product Code | Frontend Short Summary | Detailed Biological Function Description | Applicable Research Areas (comma-separated) | Targets / Pathways |
| PEP-FNAC-01 | The inactive negative-control peptide for GRGDS (a single critical Asp→Glu mutation), matching GRGDS in physicochemistry but lacking integrin binding/pro-adhesive activity; used to prove that RGD/GRGDS adhesion effects depend on the RGD sequence itself. | This is the negative-control peptide for the fibronectin RGD active fragment: the key Asp(D) of the active motif GRGDS (Gly-Arg-Gly-Asp-Ser) is the integrin-recognition core-its β-carboxylate side chain must precisely coordinate the divalent cation (Mg2+) in the integrin βA domain and the α-subunit β-propeller face, a geometry highly sensitive to distance. Replacing Asp with the one-methylene-longer Glu(E) (giving GRGES) shifts the carboxylate one -CH2- outward and destroys this precise coordination, abolishing binding to most RGD-dependent integrins (αvβ3, αvβ5, α5β1, etc.) and the associated cell adhesion/spreading activity. Because GRGES and GRGDS are nearly identical in length, charge and water solubility, differing by only one methylene, they are matched for osmolarity, non-specific adsorption and cytotoxicity-making GRGES the standard inactive control (inactive control) for RGD/GRGDS adhesion, migration, collagen-gel-contraction and angiogenesis-inhibition assays: when GRGDS produces an adhesion/inhibition effect but the same concentration of GRGES does not, the effect is proven to depend specifically on the RGD sequence rather than on non-specific peptide physicochemistry. This is the logic behind reports that GRGDS inhibits angiogenesis while GRGES does not, and that GRGDSP inhibits fibroblast spreading/gel contraction while GRGESP does not. | Cell-adhesion assay controls, RGD integrin research, fibronectin function studies, cell migration/spreading, collagen gel contraction, angiogenesis, biomaterial coating controls, in-vitro cell biology methods | RGD-dependent integrins (αvβ3, αvβ5, α5β1, etc.)-a binding-negative control that does NOT engage them; used to discriminate RGD-specific adhesion signaling from non-specific peptide effects |
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